Journal: International Journal of Molecular Sciences

Article Title: Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats

doi: 10.3390/ijms24119230

Figure Lengend Snippet: Bile-derived extracellular vesicles incorporated into the cytoplasm of the hepatic cell line (BRL-3A). Upper left: A phase contrast microscope image. Upper right: A fluorescence microscope image. Mem Dye-Deep Green: Ex 488 nm/Em 490–540 nm. Bottom: An overlay image.

Article Snippet: The cell line derived from normal rat hepatocyte cell line (BRL-3A), obtained from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan), was inoculated to a 6-well plate (1 × 10 5 cells/well), and cultured under 5% CO 2 at 37 °C for 24 h. DMEM, 10% FBS, 1% PS were used as the medium.

Techniques: Derivative Assay, Microscopy, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats

doi: 10.3390/ijms24119230

Figure Lengend Snippet: ( a ) Comparison between the PH-24 group and the sham group for genes reported to be involved in cell cycles. ( A , B ): Genes promoting the G1/S migration ( E2f3 , Dbf4 ). ( C , D ): Genes controlling the centrosome and promoting mitosis in the M phase ( Vsp4a , GEN1 ). ( E ) A gene promoting accurate chromosome segregation in the late M phase ( Chmp4c ). ( F ) A gene constantly acting on and promoting cell cycles ( Ccni ). ( G – I ) Genes whose specific functions are unclear, but are considered to promote cell cycles ( Brsk1 , Dcun1d3 , Mastl ). ( J – M ) Cell cycle checkpoint genes ( Chek2 , Nek6 , Mad2l1 , Ppm1d ). ( N , O ) Genes halting cell cycles (transitioning from G1 phase to G0 phase) ( Anxa1 , Ctdsp2 ). ( P ) A gene demonstrating the proliferative effect at the initial stage of liver regeneration ( Bcl2 ). ( Q ) A gene promoting the induction of the bile acid receptor in the hepatocyte nucleus ( Pias1 ). p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). ( b ) Upregulated genes influencing cell cycles in the PH-24 group. PH: 70% partial hepatectomy.

Article Snippet: The cell line derived from normal rat hepatocyte cell line (BRL-3A), obtained from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan), was inoculated to a 6-well plate (1 × 10 5 cells/well), and cultured under 5% CO 2 at 37 °C for 24 h. DMEM, 10% FBS, 1% PS were used as the medium.

Techniques: Comparison, Migration

Journal: International Journal of Molecular Sciences

Article Title: Role of Bile-Derived Extracellular Vesicles in Hepatocellular Proliferation after Partial Hepatectomy in Rats

doi: 10.3390/ijms24119230

Figure Lengend Snippet: EVs were added to the hepatocyte cell line (BRL-3A), and proliferative ability was verified by the MTS assay. Proliferation was significantly promoted in the PH-24 group than in the sham group and the control group. In addition, in the PH-24 group, proliferation was significantly promoted in the group with EVs added at 1.0 × 10 9 particles/well (dashed line) than in the group with EVs added at 1.0 × 10 8 particles/well (solid line). No difference was observed in the sham group and the control group. PH: 70% partial hepatectomy p < 0.001 (***).

Article Snippet: The cell line derived from normal rat hepatocyte cell line (BRL-3A), obtained from JCRB Cell Bank (Japanese Collection of Research Bioresources Cell Bank) (Osaka, Japan), was inoculated to a 6-well plate (1 × 10 5 cells/well), and cultured under 5% CO 2 at 37 °C for 24 h. DMEM, 10% FBS, 1% PS were used as the medium.

Techniques: MTS Assay, Control